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  • antoniou
    Junior Member
    • Oct 2008
    • 7

    making miRNA libraries

    Hi
    Does anyone have tried the Bioo Scientific AIR™ Small RNA Sequencing Kit? It is quite a bit cheaper than the Illumina V1.5 kit and looks very similar.

    Eric
  • link1
    Member
    • May 2010
    • 21

    #2
    We've been using the Bioo AIR small RNA Seq kit and have been very happy with it. Significantly less adapter dimers in our sequence runs with this kit compared to v1.5, not to mentioned that the Bioo kit is actually in stock !!

    Comment

    • antoniou
      Junior Member
      • Oct 2008
      • 7

      #3
      Thanks
      Do you start from total RNA or do you purify the miRNA using an acrylamide gel first?
      Eric

      Comment

      • advanT
        Member
        • Oct 2009
        • 22

        #4
        Eric, I second that. I've had good results using the kit, much better priced. For our purposes, we extract from cells and start with a very low quantity of RNA (100 ng) so we use total RNA. The guys across from us work on human miRNAs and they isolate their smalls before using this kit.

        Comment

        • link1
          Member
          • May 2010
          • 21

          #5
          We isolate our miRNA before preparing libraries. You can use any small RNA isolation reagent on the market. Trying to do it with an acrylamide gel is probably too difficult.
          Last edited by link1; 06-15-2010, 06:09 PM.

          Comment

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