making miRNA libraries

[antoniou]

Hi
Does anyone have tried the Bioo Scientific AIR™ Small RNA Sequencing Kit? It is quite a bit cheaper than the Illumina V1.5 kit and looks very similar.

Eric

[link1]

We've been using the Bioo AIR small RNA Seq kit and have been very happy with it. Significantly less adapter dimers in our sequence runs with this kit compared to v1.5, not to mentioned that the Bioo kit is actually in stock !!

[antoniou]

Thanks
Do you start from total RNA or do you purify the miRNA using an acrylamide gel first?
Eric

[advanT]

Eric, I second that. I've had good results using the kit, much better priced. For our purposes, we extract from cells and start with a very low quantity of RNA (100 ng) so we use total RNA. The guys across from us work on human miRNAs and they isolate their smalls before using this kit.

[link1]

We isolate our miRNA before preparing libraries. You can use any small RNA isolation reagent on the market. Trying to do it with an acrylamide gel is probably too difficult.