Finding differentially expressed genes between species

ishasethi7

Junior Member

Join Date: Aug 2015

Posts: 1
Share

Post
#1

Finding differentially expressed genes between species

08-26-2015, 02:12 PM

I have RNA Seq data from mouse and human skin ( 2 replicates each) and want to compare the expression of the orthologous genes to find any which are differentially expressed. I have quantile normalized the gene expression matrix across all 4 samples (2 mouse + 2 human). I eventually want to calculate the log fold change in expression of all orthologous genes between the 2 species. However before I do this, I should control for gene length, right? Will this be enough to give me an idea of the differentially expressed genes or should I be employing more sophisticated methods?

Can programs like DESeq be used for comparing gene expression between species?
Any comments would be helpful. Thanks a lot.
Tags: deseq, differential expression, quantile normalization, rna-seq

Previous template Next

Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool

by SEQadmin2

CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.¹ But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

Despite this, “CRISPR helped turn genome editing...
- Channel: Articles
07-31-2026, 11:01 AM
Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications

by SEQadmin2

Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

The systematic characterization of the human proteome has...
- Channel: Articles
07-20-2026, 11:48 AM
Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM

Topics	Statistics	Last Post
Long-Read Sequencing Closes Decades-Old Gaps in the Medaka Genome by SEQadmin2 Started by SEQadmin2, Today, 10:13 AM	0 responses 6 views 0 reactions	Last Post by SEQadmin2 Today, 10:13 AM
New Genomic Method Uncovers Ancient Hominin DNA by SEQadmin2 Started by SEQadmin2, 07-31-2026, 02:55 AM	0 responses 20 views 0 reactions	Last Post by SEQadmin2 07-31-2026, 02:55 AM
Study Captures the First Moments of DNA Replication by SEQadmin2 Started by SEQadmin2, 07-24-2026, 12:17 PM	0 responses 17 views 0 reactions	Last Post by SEQadmin2 07-24-2026, 12:17 PM
Chemotherapy Leaves Detectable DNA Signatures in Childhood Tumors by SEQadmin2 Started by SEQadmin2, 07-23-2026, 11:41 AM	0 responses 16 views 0 reactions	Last Post by SEQadmin2 07-23-2026, 11:41 AM

Unconfigured Ad

Finding differentially expressed genes between species

Latest Articles

ad_right_rmr

News