Hi, all,
We met a strange peak in prepared LncRNA library. The library construction procedure is listed below.
1 micro gram total RNA after Ribozero treatment was used at starting material and ribosomal RNA was almost completely removed as PicoRNA chip map indicated and Ribozero- RNA is ~20-50ng. Library construction was performed following NEBNext® Ultra™ RNA Library Prep Kit for Illumina protocol with size selection for 300-400bp fragments. The PCR cycle number is ~15 cycle.
In 12 of 16 samples, we found a strange sharp peak, which unlike those we usually obtained.
Did you ever meet similar results? anyone could give us an explanation?
Thanks!
attachment 1: Strange peak in library 2100 map
attachment 2: RNA after Ribozero treatment in Pico RNA map.
We met a strange peak in prepared LncRNA library. The library construction procedure is listed below.
1 micro gram total RNA after Ribozero treatment was used at starting material and ribosomal RNA was almost completely removed as PicoRNA chip map indicated and Ribozero- RNA is ~20-50ng. Library construction was performed following NEBNext® Ultra™ RNA Library Prep Kit for Illumina protocol with size selection for 300-400bp fragments. The PCR cycle number is ~15 cycle.
In 12 of 16 samples, we found a strange sharp peak, which unlike those we usually obtained.
Did you ever meet similar results? anyone could give us an explanation?
Thanks!
attachment 1: Strange peak in library 2100 map
attachment 2: RNA after Ribozero treatment in Pico RNA map.
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