Hi all, while looking for SNPs for virus quasispecies in a badnavirus using RNA that was gel-excised with maximum length of 100, maybe 200 bp I find definitive read alignment hotspots to the master genome in all 3 samples. generally people have been finding these hotspots in the populations of siRNA of 21,22 or 24 nt length, products of silencing. however the hotspots I find are in the slightly longer reads. Sequencing ran for 36 cycles, so they are 36 nt long. Also, 90+% of these reads with potential lengths between 30 and 100 nt are aligning to the sense strand. I am trying to come up with an explanation for these two things and drawing a blank. I am not a virologist or even microbiologist, so am I missing something obvious? I apologize if this is not the place to ask this question, and would appreciate any answers or other places to ask this question.
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...