Tweet
Hi all, while looking for SNPs for virus quasispecies in a badnavirus using RNA that was gel-excised with maximum length of 100, maybe 200 bp I find definitive read alignment hotspots to the master genome in all 3 samples. generally people have been finding these hotspots in the populations of siRNA of 21,22 or 24 nt length, products of silencing. however the hotspots I find are in the slightly longer reads. Sequencing ran for 36 cycles, so they are 36 nt long. Also, 90+% of these reads with potential lengths between 30 and 100 nt are aligning to the sense strand. I am trying to come up with an explanation for these two things and drawing a blank. I am not a virologist or even microbiologist, so am I missing something obvious? I apologize if this is not the place to ask this question, and would appreciate any answers or other places to ask this question.
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM