Can i do RNA SEQUENCING without having the genome?
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Yes, you can. -
Can you recommend me where to begin?Comment
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It's hard to be specific without knowing what you want to do, but generic advice wouild be to start by reading this review paper:
Then go on to more specialized papers, or search this forum, for more information about "transcriptome assembly".Comment
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RNA-SEQ with no genome
Two recent papers discuss methodology and strategy:
De novo assembly and analysis of RNA-seq data - Nature Methods 2010
and a News & Views Article from Nature Biotechnology 2010
Advancing RNA-Seq analysis - New methods for analyzing RNA-Seq data enable de novo reconstruction of the transcriptome
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thanks mates!
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