Can i do RNA SEQUENCING without having the genome?
-
-
Yes, you can. -
Can you recommend me where to begin?Comment
-
It's hard to be specific without knowing what you want to do, but generic advice wouild be to start by reading this review paper:
Then go on to more specialized papers, or search this forum, for more information about "transcriptome assembly".Comment
-
RNA-SEQ with no genome
Two recent papers discuss methodology and strategy:
De novo assembly and analysis of RNA-seq data - Nature Methods 2010
and a News & Views Article from Nature Biotechnology 2010
Advancing RNA-Seq analysis - New methods for analyzing RNA-Seq data enable de novo reconstruction of the transcriptome
Comment
-
thanks mates!
Comment
-
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment