Can i do RNA SEQUENCING without having the genome?
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It's hard to be specific without knowing what you want to do, but generic advice wouild be to start by reading this review paper:
PubMed® comprises more than 36 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full text content from PubMed Central and publisher web sites.
Then go on to more specialized papers, or search this forum, for more information about "transcriptome assembly".
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RNA-SEQ with no genome
Two recent papers discuss methodology and strategy:
De novo assembly and analysis of RNA-seq data - Nature Methods 2010
and a News & Views Article from Nature Biotechnology 2010
Advancing RNA-Seq analysis - New methods for analyzing RNA-Seq data enable de novo reconstruction of the transcriptome
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
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