Hi I have a problem when run tophat. I have recieved this massage:
Error running bowtie:
Warning: output file '-' was specified without -s. This will not work in future Bowtie 2 versions. Please us -s instead.
Warning: could not open read file "/1AM21/tophat1/tmp/index" for reading; skipping...
Error: no input read files were valid.
(ERR): bowtie2-align exited with value 1
Please help me!!!
Also, the attached link is the picture of my commend!
Error running bowtie:
Warning: output file '-' was specified without -s. This will not work in future Bowtie 2 versions. Please us -s instead.
Warning: could not open read file "/1AM21/tophat1/tmp/index" for reading; skipping...
Error: no input read files were valid.
(ERR): bowtie2-align exited with value 1
Please help me!!!
Also, the attached link is the picture of my commend!