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Did anyone has the idea of what changes in True seq mRNA/smallRNA sample prep kit by Illumina?
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TruSeq™ Sample Prep Kit and Cluster Kit Compatibility
TruSeq Kit Compatibility Product Insert (15017916)[1].pdf
I'm interested in the details of this as well, specifically because we're doing R & D for non-bias amplification with bar-coding. We got there...and now they are "phasing out" the flow cells that go with what we have working (grr).
Anyway, this is as far as we can get out of them for now: TruSeq™ Sample Prep Kit and Cluster Kit Compatibility. I'm sure as soon as someone drop clones the prepped sample and has it sequenced, Illumina will release the sequence (it's not like we all can't figure it out).
Good luck, and I encourage anyone who gets there first to sequence the bits and share the info! I will sure do so. -
Illumina will be releasing sequences for Tru-Seq, at least that's what they told us in a seminar. Oligo only also sonds like it wil be coming but how many people out there really want the hassle of creating DIY kits. It does not look like many £ or $ wil be saved. Of course if you are developing a protocol the kits might not be the right thing to use.
The Tru-Seq RNA sampe prep kit does not look like it does strand-specific so I guess they might still recommend their own experimental protocol using the smallRNA kits.
Can anyone say why the Parkhomchuk method would not be possible with a modified Illumina Tru-Seq RNA sample prep kit?Comment
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Well for large projects using automation DIY kits are always going to be the way to go.Comment
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They said they will eventually release the strand specific protocol but having used the new TruSeq kits for small RNA and mRNA it is pretty easy to just modify the existing protocol with the new kits to generate strand specific libraries.Originally posted by james hadfield View PostComment
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Truseq mRNA but strand specific
Hey mnkyboy, how would that modification look like then?Originally posted by mnkyboy View Post
I am pretty new in the field, meaning I just started to try establishing RNA seq on plant material. however, and would like to get some strand specificity?
Would be great if you could suggest a combination....meaning something as a mix of the Truseq mRNA kit and the Parkhomchuk protocol.
Thank you in advance!Comment
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Parkhomchuk combined with Illumina Tru-Seq RNA
Can anyone say why the Parkhomchuk method would not be possible with a modified Illumina Tru-Seq RNA sample prep kit?[/QUOTE]
I would like to know as well indeed?
Would be nice to help me out!
Kind regards!Comment
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I could not get a straight answer about this from Illumina after I went through the newer protocols and it was not that straightforward. They said it is coming soon though.Originally posted by mcseq View Post
For our strand specific we are still using Illumina's original protocol using their older mRNA and small RNA kits and it is working very well for strand specificity.Comment
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Truseq mRNA but strand specific
Thanx mnkyboy, then I have to be patient and start a trial for the combination right away!
All the best!Comment
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The Parkhomchuk method incorporates Uracil that is then degraded at a later step. My understanding is that there is IP around this that would prevent companies from releasing kits without paying licence fees. It should work fine.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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