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  • zizi
    Junior Member
    • Feb 2019
    • 1
    #1

    New to RNA seq.. Need help!!!

    Hello All

    I am doing RNA seq using KAPA Stranded RNA-Seq Kit with RiboErase. I am following all the recommendations of the protocol but I seem to lose all my RNA during rRNA depletion step


    Please suggest what to do.
    Tags: None
    • luc
      Senior Member
      • Dec 2010
      • 469
      #2
      How do you know? I would suggest to follow the library prep protocol all the way to the end and then assess the library or lack thereof.

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