Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Tsequencing
    Junior Member
    • Dec 2018
    • 5

    Control-based correction in multispecies RNAseq comparison

    Hi all!

    I think this issue fits better here than on another forum I posted this question some time ago:

    In a multispecies gene expression comparison (per species one control group and 3 treatments) I want to compare the gene expression patterns of specific gene families. For this I want to correct the expression levels of these genes per species using the control group. In this way you get the percentage difference in expression of the genes for each treatment in comparison to the control treatment, and I can compare the gene expression levels of the different species directly instead of only looking at within species patterns and comparing both.

    I have not seen a similar approach in other multi-species RNAseq studies. Are there perhaps similar studies or papers dealing with a multispecies comparison of RNAseq data that I have overlooked?

    Like to hear your opinion!
  • Gopo
    Member
    • Nov 2013
    • 41

    #2
    Sounds logical, but not sure. This article might be of interest...

    Comment

    • Tsequencing
      Junior Member
      • Dec 2018
      • 5

      #3
      Ah! Thanks for the suggestion, I will give it a try.

      Comment

      • INTENSE X
        Junior Member
        • Apr 2019
        • 1

        #4
        thank you will try it
        intense x reviews best male enhance pills reviews

        Comment

        Latest Articles

        Collapse

        • GATTACAT
          Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by GATTACAT
          Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
          07-01-2026, 11:43 AM
        • SEQadmin2
          Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by SEQadmin2


          I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

          Here are nine questions we think about, in roughly the order they matter, before...
          06-18-2026, 07:11 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 07-02-2026, 11:08 AM
        0 responses
        10 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-30-2026, 05:37 AM
        0 responses
        13 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-26-2026, 11:10 AM
        0 responses
        20 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-17-2026, 06:09 AM
        0 responses
        54 views
        0 reactions
        Last Post SEQadmin2  
        Working...