Greetings RNA-Seq'ers,
I am preparing samples for RNA-seq, and my final PCR enrichment product contains dimers and trimers of my 350bp fragments (~700bp and ~1000bp). I have gel extracted the PE product at 350bp, but the BioAnalyzer still shows the the dimers and trimers. Does anyone know how it is possible that the longer fragments are running at the same speed as the 350bp fragments on the gel? Any ideas on how I can remove the dimers from my PE product?
Thanks a Bunch!
Melanie
I am preparing samples for RNA-seq, and my final PCR enrichment product contains dimers and trimers of my 350bp fragments (~700bp and ~1000bp). I have gel extracted the PE product at 350bp, but the BioAnalyzer still shows the the dimers and trimers. Does anyone know how it is possible that the longer fragments are running at the same speed as the 350bp fragments on the gel? Any ideas on how I can remove the dimers from my PE product?
Thanks a Bunch!
Melanie
Comment