Hi everyone
We performed an RNA-seq experiment using small RNAs in a species with no genome sequence. I've used miRProf to find which microRNAs are expressed in a control sample (3 replicates) and in a stressed sample (3 replicates).
Now I would like to calculate the Fold Change between the two conditions, but I have no idea about the expected inter-replicates variability. Which criteria should I use to filter out expression values with too much difference between the replicates?
Thanks
Moreno
We performed an RNA-seq experiment using small RNAs in a species with no genome sequence. I've used miRProf to find which microRNAs are expressed in a control sample (3 replicates) and in a stressed sample (3 replicates).
Now I would like to calculate the Fold Change between the two conditions, but I have no idea about the expected inter-replicates variability. Which criteria should I use to filter out expression values with too much difference between the replicates?
Thanks
Moreno
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