Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • plefebvre
    Member
    • Jan 2010
    • 10

    #1

    Cufflinks then Cuffdiff

    Hi there
    I have ran a RNA-seq on Illumina GII, and processed the bam files on the Galaxy server. Everything ran smoothly untill I tried to run Cuffdiff on may assembled transcript files which always yields an error as this

    566: Cuffdiff on data 492, data 493, and data 521: CDS diff
    An error occurred running this job: cuffdiff v0.9.3 (1649)
    cuffdiff -q -p 8 -Q 0 -c 1000 --FDR 0.050000 -N -r /galaxy/data/mm9/sam_index/mm9.fa /galaxy/main_database/files/002/338/dataset_2338625.dat /galaxy/main_database/files/002/337/dataset_2337232.dat /galaxy/main_database/files/002/337

    Has somebody ever ran into this kind of problem (the Glaxy team sais this is a Cufflinks problem, but no answers yet from those guys ...)
    Thanks
  • honey
    Senior Member
    • Feb 2010
    • 151

    #2
    error in Cuffdiff

    It appear that you are running on Galaxy you may like to post your question on their mailing list and may be there is some glitch in Galaxy

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Working...