primer/adapter sequences

[nikiwilson]

Hi,
can anyone please help??? I have been using the Illumina ChIP-Seq amplification kit for a while now and would like to move to the NEB kit. The NEB kit doesn't contain the primers/adapters and they suggested looking on this forum for the sequences, can anyone help???? Where would you normally order them from?? Do they need to be HPLC purified????
Many thanks

[liupf56]

See the attached file. Use PE adapters and PE PCR primers on page 3. You can order them from IDT and choose PAGE purification.

[NextGenSeq]

Note that for best results the bond between the last 2 bases on the 3' end must be a phosphothioate bond. Most oligo companies can do this. If you don't modify the oligo you will get adapter artifacts.