Hi All
I'm trying to isolate DNA from large volumes of plasma (anywhere from 10 to 50mL) in order to sequence it. I've been using Norgen's Circulating DNA Isolation kit but my yields have been very low so far, about 1.8ng of DNA per 5mL of plasma. Does anyone have any experience with either the Norgen kit or isolating nucleic acid from such large volumes?
Thanks!
I'm trying to isolate DNA from large volumes of plasma (anywhere from 10 to 50mL) in order to sequence it. I've been using Norgen's Circulating DNA Isolation kit but my yields have been very low so far, about 1.8ng of DNA per 5mL of plasma. Does anyone have any experience with either the Norgen kit or isolating nucleic acid from such large volumes?
Thanks!
Comment