Hello,
I have recently received the results of my ChIP Seq data, prior to amplification I verified that the ChIP had worked using a positive control region (vs a negative control region) with qpcr. The sequencing data shows no peak in this region and actually the data looks like the input control sample (with just random reads across the genome). I am sure that the ChIP worked so there must be something wrong with the library prep or the sequencing, has anyone else had this problem?
Thanks
Chloe
I have recently received the results of my ChIP Seq data, prior to amplification I verified that the ChIP had worked using a positive control region (vs a negative control region) with qpcr. The sequencing data shows no peak in this region and actually the data looks like the input control sample (with just random reads across the genome). I am sure that the ChIP worked so there must be something wrong with the library prep or the sequencing, has anyone else had this problem?
Thanks
Chloe
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