miR seperation

[swarner]

Hello,

I am working with a customer interested in building a miR library. In the past we have always used flash page fractionators to separate the miRs from the rest of the RNA. Flash page fractionators have been discontinued, what is everyone else using for separation?

[Deli Çoban]

Hi,

1) Separate total RNA on a 15% denaturing PAGE (7M Urea) for 90 minutes at 275V.

2) stain the gel with GelStar™ (it works pretty well but, I also tried by using Et. Bromide)

3) Select RNA fragment(s) to be purified and excise them from the gel.

4) Just the purify the desired band from gel (any kits)

easy to come!

[swarner]

Thanks, I'll pass that along