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My facility has recently purchased an Illumina GA IIx. I was wondering for qPCR during sample prep, what kits/reagents people recommend? We have an ABI stepOnePlus instrument.
Thanks!
Thanks!
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KAPA kit I would recommend -
If you're looking for a SYBR (or similar)-based master mix, I can tell you that Fisher Scientific has a promotional deal on Promega's GoTaq qPCR mix that is ridiculously cheap. It is sold as 1000 or 200 reactions, but that's based on a 50uL reaction volume, so if you do 25uL reactions, that's the price you'd get for 2000 or 400 reactions.
GoTaq qPCR promoComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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