Hello
I am struggling with mRNA extraction from frozen tissues.
Unlike fresh tissue, frozen tissue includes lots of degraded rRNAs.
Although FAS from SOLiD recommended to use poly-A capture, I'd like to prepare mRNA from total RNA by removing rRNAs.
I was told that Ribo-Zero was much better than Ribominus to remove rRNA.
Anyone has experience with Ribo-Zero?
I am struggling with mRNA extraction from frozen tissues.
Unlike fresh tissue, frozen tissue includes lots of degraded rRNAs.
Although FAS from SOLiD recommended to use poly-A capture, I'd like to prepare mRNA from total RNA by removing rRNAs.
I was told that Ribo-Zero was much better than Ribominus to remove rRNA.
Anyone has experience with Ribo-Zero?
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