[riehle]

NEXTflex Chip-Seq Kit and qiagen columns

Hi
I wonder if anybody has experience in replacing the AmpureBeads with Qiagen Columns for lib prep using the NEXTflex Chip-Seq Kit.
Thanxx

[RNAseqer]

We are using Bioo's ChIP-Seq kit with beads. I believe you have to use beads because the adapters are long and cannot be removed by columns.

[Devero89]

looks good so far. I will seq the multiplexed lib. that I made with Bioo.

[james hadfield]

Our first four libraries will go on next week, fingers crossed as we'd like to move to 96-plex.

[Devero89]

it worked!!

[bluescapebioo]

thanks ,I learn a lot

[bluescapebioo]

thanks a lot,

[njina]

Quality of sequecning data from NEXTFlex

For those of you who have used NEXTFlex to prepare libraries, can you comment on the quality of the sequencing data?