Truseq RNA kit for making DNA library

[elisadouzi]

I want to make 500bp paired-end library from genomic DNA. I already have the Truseq RNA kit. I only have two samples and don't want to order the expensive Truseq DNA kit. I check the manual and wandering maybe we can use the RNA kit from end repair step after sonicating. Does anyone try this?

Thanks a lot!

[Zahra-A]

Although your post is from long ago, I was wondering how you finally ended up. I am in a similar situation now.
I read that the adaptors in the RNA and DNA TruSeq kit are the same, though the concentrations are not.

[Manna]

It works totally fine. I've done it. The two kits a indistinguishable once you have clean dsDNA. The primers and reaction mixes are at the same concentrations.

[Riva]

We did use RNA kit for all of our RNA/DNA cases. They were doing well and we could happily maximize the use of the RNA kit.