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I found this link thoroughly describing how to prepare mRNAseq libraries from only 10 ng of total RNA and I wondered if anybody here ever tried something like that.
Are such protocols reliable or should I stick with a kit (e.g. Ovation's RNAseq kit)?
And another general question for those who prepared RNAseq libraries by linear amplification. What measures would you recommend to prevent contaminations before the amplification? All kit manuals I've read so far are extremely concerned and suggest to use a seperate lab for pre-amplification steps.
Are such protocols reliable or should I stick with a kit (e.g. Ovation's RNAseq kit)?
And another general question for those who prepared RNAseq libraries by linear amplification. What measures would you recommend to prevent contaminations before the amplification? All kit manuals I've read so far are extremely concerned and suggest to use a seperate lab for pre-amplification steps.