Hi all
Has anyone any experience of preparing 454 rapid libraries after shearing with the Covaris?
We've generally used the 400bp settings (Duty Cycle:10%, Intensity:4, Cycles/burst:200, Time:55 seconds) before and produced excellent 700bp libraries. However, recently we've seen out libraries come out at lower yield and around 1kb.
Does anyone have any optimised settings for Covaris shearing on genomic DNA?
Thanks
Tony
Has anyone any experience of preparing 454 rapid libraries after shearing with the Covaris?
We've generally used the 400bp settings (Duty Cycle:10%, Intensity:4, Cycles/burst:200, Time:55 seconds) before and produced excellent 700bp libraries. However, recently we've seen out libraries come out at lower yield and around 1kb.
Does anyone have any optimised settings for Covaris shearing on genomic DNA?
Thanks
Tony
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