Library prep for 50ng input with TruSeq DNA- or RNAseq adapters?

[sehrrot]

Dear all,

I am going to process library preparation with 50 ng DNA. I am just concerning ratio of adaptersNA input. Thus, I am considering to use adapters from TruSeq RNAseq kit, which has lower molarity adapters. Has anyone done library prep with lower DNA input?

[pmiguel]

Yes or you could just dilute your DNA TruSeq adapters 20x.

We have gone below 1 ug. Possibly to 100 ng, with no modifications to the protocol. But we generally size select prior to enrichment PCR. So adapter dimers are not that much of an issue.

--
Phillip