We are trying to troubleshoot contamination issues that we are having in our ChIP-seq experiments. It seems that recently we have been sequencing constructs that have been cloned in the lab in other experiments in our IPd samples. We are trying to decided how to proceed, how many precautions to take. We are interested in hearing what others do. Do you have a designated area, designated equipment, etc.? At what point in the procedure do you begin to use this designated area? As early as the IP or just at the amplification step? Any and all of your thoughts and suggestions would be greatly appreciated!
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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