We are looking a making a set of barcoded adapters for Illumina sequencing. We know the sequences already but I wanted to ask people who have done it if they incorporated any modifications in the oligos. Specifically the use of phosphorothioate linkages. I'm asking because Roche/454 recently released their protocols and adapter sequences for doing MID tag sequencing on Titanium and they call for phosphorothioate links at the first and last 4 bases of each oligo.
I would appreciate hearing from people who have made their own adapters for Illumina sequencing. Did you use modified linkages? What were your experiences with homemade or custom adapters? Any lessons learned would be greatly appreciated. Thanks in advance.
I would appreciate hearing from people who have made their own adapters for Illumina sequencing. Did you use modified linkages? What were your experiences with homemade or custom adapters? Any lessons learned would be greatly appreciated. Thanks in advance.
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