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Library quality check using known genes
Hi Everyone,
I'm hoping someone can give me advice on quality control of the library obtained using the Illumina protocol.
I have a set of genes that I use to check by qPCR when I'm doing the ChIP and then later in the final library. I'wondering if I have to expect the some enrichment (the numbers of cycles at the which they come out) in the original ChIP and in the library, or if I've to expect even to not see some genes that came in the later cycles.
I'm mean I should expect the same sensibility between the qPCR machine and the ChIP-Sequencing?
Is possible that the sequencing can reveals those genes that are not revealed by qPCR-machine?
Many thanks!!
I'm hoping someone can give me advice on quality control of the library obtained using the Illumina protocol.
I have a set of genes that I use to check by qPCR when I'm doing the ChIP and then later in the final library. I'wondering if I have to expect the some enrichment (the numbers of cycles at the which they come out) in the original ChIP and in the library, or if I've to expect even to not see some genes that came in the later cycles.
I'm mean I should expect the same sensibility between the qPCR machine and the ChIP-Sequencing?
Is possible that the sequencing can reveals those genes that are not revealed by qPCR-machine?
Many thanks!!