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I am planning to deep sequence material from 13 samples. For this, I am isolating RNA from various organs, reverse transcribing with a gene specific primer and performing a multiplex PCR (all products are ~400 bp). Then, I will ligate a barcode to the PCR product from each sample and sequence. Since I am jumping into the middle of the standard 454 prep, can someone please tell me how much starting material I need for each sample for a full 454 plate?
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM