HiC - crosslinking - glycine

sebbol

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Join Date: Jan 2012

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HiC - crosslinking - glycine

01-12-2012, 06:05 PM

hello. i have 2 questions.

1. what would be the best way to evaluate if the cross-linking went okay for Hi-C (chromosome interactions)?

2. i read different things about the temperature of quenching the formaldehyde with glycine. some do it at RT, some do it on ice or with ice-cold glycine. i am not a chemist. why would the temperature be of importance?

thanks for reading the post and your kind help!

Seb
Tags: crosslinking, formaldehyde, glycine, hic

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