Presence of RNA for Sureselect XT

[moedog45]

Hello,

I have some gDNA samples that have a sufficient amount of DNA according to the Qubit fluorometer and also have a 260/280 ratio of about 1.85-1.90. However, I'm concerned that there may still be some RNAs in these samples because they were not treated with RNAse during sample prep. Would the presence of RNA seriously impact any aspect of the SureSelect XT protocol? If so, are there any suggestions about how best to treat these samples with RNAse?

[liliniu]

Hi moedog45:

You may contact SureSelect support for your question.

Here are the contact information:
SureSelect Support
Phone – 800-227-9770 x 3 x 4 x 4 (North America) Email – [email protected]

Hello,

I have some gDNA samples that have a sufficient amount of DNA according to the Qubit fluorometer and also have a 260/280 ratio of about 1.85-1.90. However, I'm concerned that there may still be some RNAs in these samples because they were not treated with RNAse during sample prep. Would the presence of RNA seriously impact any aspect of the SureSelect XT protocol? If so, are there any suggestions about how best to treat these samples with RNAse?