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  • Janiz
    Junior Member
    • Mar 2012
    • 2
    #1

    NGS library prep on crosslinked chromatin

    Hi there,

    I'm new here and to NGS and hopefully you guys can help me

    Has anyone NGS library preps on crosslinked chromatin? I am having some issues with the ligation of the Illumina adapters to my chromatin, any suggestions?

    I am currently using the NEB T4 ligase, a 1:10 dilution of the adapters and between 1-10 ug of chromatin. Similar to the HiC method I digest my chromatin and fill in the overhangs using Klenow (exo).

    Thanks!
    Tags: None
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