I'm a beginner! Help?

[smadbro]

Hi guys, I'm a beginner I'm trying to understand next gen sequencing. Basically I'm writing a project up in which I extracted DNA/RNA for next generation sequencing. I'm writing at why I would require high molecular weight DNA/RNA in order to construct libraries. I don't really understand what libraries this means? I think it could mean the sheared fragments that are clonally amplified before using NGS on them, but I'm very stuck. Are there any papers you could link me to which would make this sort of thing clearer?

Thanks alot!

[HESmith]

Most protocols explain the rationale for using high MW material; that would be a good place to start.

[jeev]

What would the explanation be? I've not seen a direct reason the protocols suggesting it so far.

[marcowanger]

Hi guys, I'm a beginner I'm trying to understand next gen sequencing. Basically I'm writing a project up in which I extracted DNA/RNA for next generation sequencing. I'm writing at why I would require high molecular weight DNA/RNA in order to construct libraries. I don't really understand what libraries this means? I think it could mean the sheared fragments that are clonally amplified before using NGS on them, but I'm very stuck. Are there any papers you could link me to which would make this sort of thing clearer?

Thanks alot!

For general idea: http://en.wikibooks.org/wiki/Next_Ge...quencing_(NGS)