I am new to this forum, so I apologize if someone has asked this question already. I have been prepping total RNA from rat liver tissue using the miRNeasy kit from Qiagen and analyzing the RNA using the Agilent pico kit. My RIN values are averaging in the 2.3 range and I understand that the RIN value should be ~8. I was just wondering if others have used the same method of total RNA isolation from flash frozen tissue and what RIN values you typically see? Thanks for any help!
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
