Anyone had issues with a Covaris sonicator cracking tubes? We are using the microTUBE AFA Fiber Pre-Slit Snap-Cap 6x16mm in a Covaris S2 that shipped with our SOLiD years ago.
No problems with it until a month ago when we noticed that the waterbath degasser was not functioning. Applied Biosystems FSE came and replaced the degasser pump motor. Seemed to be fine, except that about 1 in 3 of the micro tubes are cracking at the neck. Obviously not good. We have tried different lots of tubes -- same thing. So I presume it is an instrument issue.
Also, the 150 bp Covaris protocol seems to be shearing to ~75 bp.
Any ideas?
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Phillip
No problems with it until a month ago when we noticed that the waterbath degasser was not functioning. Applied Biosystems FSE came and replaced the degasser pump motor. Seemed to be fine, except that about 1 in 3 of the micro tubes are cracking at the neck. Obviously not good. We have tried different lots of tubes -- same thing. So I presume it is an instrument issue.
Also, the 150 bp Covaris protocol seems to be shearing to ~75 bp.
Any ideas?
--
Phillip
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