Anyone sequencing mate pair libraries beyond the recommended 36bp?
Thanks,
Phillip
Thanks,
Phillip
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I was thinking about doing the same thing as well, has anyone tried this ?Originally posted by PNG View Post
Thanks -
Mate pair length
I recently did a 101-bp run with indexed mate pairs. Rather than cut out a 300-400 bp chunk at the second fragmentation step, I took a 600 bp chunk. So far, we have not seen any odd bioinformatic results indicating a junction-point issue. That said, you can always just trim the ends if it is of concern. I indexed the mate pair and had it in with 4 other bugs that I prepared as paired end. The mate-pair sample definitely was the cleanest of the group, i.e. better quality scores toward the end of the reads.Comment
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