Total RNA from lymph node

whw

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Join Date: Jun 2011

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Total RNA from lymph node

06-05-2012, 01:26 PM

Hi All,

I'm working on an RNA sequencing protocol starting from lymph nodes stored in RNAlater but my yields of total RNA have been disappointing–3-5ug from <50mg desiccated tissue. Does anyone have experience with RNAlater samples? What kind of yields should I expect?

I'm using the TissueRuptor with RNAzol RT (MRCgene) for precipitation of total RNA.

thanks,
Will
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