I was hoping that someone might know of a good probe design software. My method is sort of based off of the oligo ligation assay and MLPA but it's not exactly either of those. I just want to be able to have something that if I input a sequence with a SNP, it will return the upstream hybridization oligo and downstream hybridization oligo to a SNP site. I have attached a photo to describe what I mean. I realize this might not be the best forum to post this in, but I don't know where else to ask. My goal would be to make hybridization oligos within a specific size range, close Tm, and 50/50ish GC content. I can do this by hand for a couple but I would like to expand my project to a couple of hundred sites. If anyone has any program suggestions I would appreciate it. Ideally something a dummy (like me) could use or at least a program with some tutorials. I have looked into Probemaker but even that seems awfully complicated. Even if the program isn't free I would look into it.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
