Tweet
I was hoping that someone might know of a good probe design software. My method is sort of based off of the oligo ligation assay and MLPA but it's not exactly either of those. I just want to be able to have something that if I input a sequence with a SNP, it will return the upstream hybridization oligo and downstream hybridization oligo to a SNP site. I have attached a photo to describe what I mean. I realize this might not be the best forum to post this in, but I don't know where else to ask. My goal would be to make hybridization oligos within a specific size range, close Tm, and 50/50ish GC content. I can do this by hand for a couple but I would like to expand my project to a couple of hundred sites. If anyone has any program suggestions I would appreciate it. Ideally something a dummy (like me) could use or at least a program with some tutorials. I have looked into Probemaker but even that seems awfully complicated. Even if the program isn't free I would look into it.
-
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM