[lailaizhang]

Below 100ng then HS, 100ng-1ug then BR.
It's also good practice to re-QC normalised samples too.

Thanks. Looks like I should use the HS kit since my DNA is only about 5 ng/ul

[subxero]

Thanks. Looks like I should use the HS kit since my DNA is only about 5 ng/ul

it's pointed out in another thread somewhere but if you are switching between the 2 kits you need to do some comparison tests of your samples using each kit. BR tends to give a higher reading by a high percentage vs the HS in my experience.

I once ran out of the BR dsDNA assay which I had been using to measure the concentration of my RNAseq libraries for cluster concentration calculations and instead used a HS assay in a pinch, did the calculations the same and clustered at "the same" concentration and all my libraries over clustered. I then did a side by side of the libraries with the BR and HS and there was on average a 29% difference between the values given, with the BR being higher. All the libraries were falling in the 40ng/ul range on the HS so they were well with in the BR kit range

I had good consistent clustering results with the BR assay when using it, and only had a problem with it when i switched to HS without making adjustments for the different values given. (<-- unknown to me at the time, or an oversight) I will stick with HS from now on for anything under 100ng/ul, I do feel like it is more accurate with in that range vs the BR