I was wondering what conditions give the best elution efficiencies from dynal magnetic streptaviden beads. According to the invitrogen (http://www.invitrogen.com/site/us/en...eptavidin.html), the best buffers seem to include 95% formamide. However, I would like to purify with Ampure after the elution. I contacted Beckman Coulter and they said that formamide removal wasn't tested. The protocol I am using now has 3 min incubation at 95oC in water to break the streptaviden-biotin bond. Does anyone have any recommendations from their experience with these beads?
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