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I was wondering what conditions give the best elution efficiencies from dynal magnetic streptaviden beads. According to the invitrogen (http://www.invitrogen.com/site/us/en...eptavidin.html), the best buffers seem to include 95% formamide. However, I would like to purify with Ampure after the elution. I contacted Beckman Coulter and they said that formamide removal wasn't tested. The protocol I am using now has 3 min incubation at 95oC in water to break the streptaviden-biotin bond. Does anyone have any recommendations from their experience with these beads?
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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