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  • boxndragon04
    Junior Member
    • Feb 2012
    • 1

    Denaturing

    Has anyone tried Denaturing with NaOH and then followed by a heat denaturing?

    In other words do both?

    Does that help make better clusters??

    Running a MiSeq
  • pmiguel
    Senior Member
    • Aug 2008
    • 2328

    #2
    I would not recommend it. Either method will take its toll on the library molecules. Both seems like over-kill anyway.

    On the other hand, I have mixed NaOH denatured (phiX) with heat denatured (NexteraXT) libraries, just before starting a run. That worked fine.

    --
    Phillip

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