Hi everyone
We are using a library preparation method that involves cutting the genomic DNA with an enzyme that should cut about every 10 kb. We would like to check if the digestion worked but when running a gel with the genomic DNA and the digested DNA next to it we cannot see much. The main reason is that the genomic DNA is already quite fragmented and does not show up as a nice band.
Does anyone know a more accurate way to check the quality of genomic and enzyme digested DNA than running it on an agarose gel? Something like a Bioanalyzer would be best, but there are only Bioanalyzer chips for DNA up to 12 kb length.
Thanks in advance for any ideas.
Cheers,
Joana
We are using a library preparation method that involves cutting the genomic DNA with an enzyme that should cut about every 10 kb. We would like to check if the digestion worked but when running a gel with the genomic DNA and the digested DNA next to it we cannot see much. The main reason is that the genomic DNA is already quite fragmented and does not show up as a nice band.
Does anyone know a more accurate way to check the quality of genomic and enzyme digested DNA than running it on an agarose gel? Something like a Bioanalyzer would be best, but there are only Bioanalyzer chips for DNA up to 12 kb length.
Thanks in advance for any ideas.
Cheers,
Joana