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  • #1

    unusual library peaks

    Hello there

    I've made 2 new libraries out of totalRNA, with the Illumina tru-seq kit and manual.

    I've tested them both on the Bioanalyzer.
    The ladder seems to be good and the main peak is ok but I don't know why I have all those little peaks in the range between 100-400 bp.
    Does anyone know why?
    I thought that maybe they could be adapter-dimers, but why do they run higher than 150bp?





    thanks
    Last edited by Steffen_L; 11-09-2012, 01:45 AM.
    Tags: None
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