We want to use the ScriptSeq v2 RNA-seq Library prep kit (Epicentre) to make libraries for some rRNA-depleted bacterial RNA. Looking through the protocol I don't see any option for a size-selection. We'd like to size-select because we have a lot of small RNA product that we don't want to include in the prep. Has anyone incorporated a size-selection into the protocol? I would think you could add it after the completed cDNA synthesis and tagging (after 3.C in current protocol). Would you still do the cDNA purification (3.D in current protocol) if there is a size-selection after cDNA synthesis? We have access to a pippin prep if that will be useful.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...Yesterday, 05:41 AM