We want to use the ScriptSeq v2 RNA-seq Library prep kit (Epicentre) to make libraries for some rRNA-depleted bacterial RNA. Looking through the protocol I don't see any option for a size-selection. We'd like to size-select because we have a lot of small RNA product that we don't want to include in the prep. Has anyone incorporated a size-selection into the protocol? I would think you could add it after the completed cDNA synthesis and tagging (after 3.C in current protocol). Would you still do the cDNA purification (3.D in current protocol) if there is a size-selection after cDNA synthesis? We have access to a pippin prep if that will be useful.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM