trouble with RNA seq library preparation
hi,
i am doing RNA seq library for the first time , and used the protocol from
http://www.frontiersin.org/Plant_Gen...00202/abstract (something which works beautifully for the lab guys who developed it)
i repeated the protocol twice and never got any result. the gel (after enrichment step) just showed a faint band of primer dimers. can anyone tell me where am i going wrong? could it be a simple case of not drying the beads enough after the ethanol wash? at what stages can i check if the steps have worked?
thanks
suja
i am doing RNA seq library for the first time , and used the protocol from
http://www.frontiersin.org/Plant_Gen...00202/abstract (something which works beautifully for the lab guys who developed it)
i repeated the protocol twice and never got any result. the gel (after enrichment step) just showed a faint band of primer dimers. can anyone tell me where am i going wrong? could it be a simple case of not drying the beads enough after the ethanol wash? at what stages can i check if the steps have worked?
thanks
suja