Hey Guys,
I'm rather new to library construction & sequencing and am unsure of a few things. A little background: I'm using the Truseq sample preparation kit for library workflow, and my colleague is using the KAPA Biosystems Library preparation kit. We're doing low throughput construction (about 5 libraries per construction) and I'm wondering what format is generally best to use as the tube and magnets go. I've been reading that some people use 1.5 mL microcentrifuge tubes and others use PCR tubes both with their corresponding magnets. PCR tubes fit into our thermocycler for controlled incubations while 1.5 mL tubes do not, does everyone follow the exact incubation temperatures? Whats the best approach to deciphering what format that I should use? Or what is the most common tube/magnet used?
I'm rather new to library construction & sequencing and am unsure of a few things. A little background: I'm using the Truseq sample preparation kit for library workflow, and my colleague is using the KAPA Biosystems Library preparation kit. We're doing low throughput construction (about 5 libraries per construction) and I'm wondering what format is generally best to use as the tube and magnets go. I've been reading that some people use 1.5 mL microcentrifuge tubes and others use PCR tubes both with their corresponding magnets. PCR tubes fit into our thermocycler for controlled incubations while 1.5 mL tubes do not, does everyone follow the exact incubation temperatures? Whats the best approach to deciphering what format that I should use? Or what is the most common tube/magnet used?
Comment