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Hi, anybody here currently working with the new Hydroshear Plus form Digilab? I am in a big problem here!
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...and your problem is? -
hydroshear plus
i have followed as instructed in the manual. the same speed code & cycle. however, the syringe broke 2 times already. after changing the syringe, i successfully perform the shearing. when i wanted to repeat the shearing, i notice there is white precipitate inside the syringe. now i don't know what should i do. and i think the syringe broken again.
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Hope you managed to solve your problem in the meantime, but if not...
Do you wash the system often enough between and after shearing with filtered solutions (water, HCl, NaOH)?
Did you make sure your shearing assembly is not clogged? Look at the assembly under a microscope to make sure it is not clogged or damaged; in any case try washing it by putting in a sonicating water bath with distilled water or a weak SDS solution.
To make sure your assembly does not get clogged, make sure your DNA is super pure! The white precipitate could possibly be salt. Do a buffer exchange with columns or other purification means! If there are particles in your DNA sample or the DNA is not completely solubilized, it can clog the shearing assembly.
If the assembly is clogged the syringe can break.
We've only managed to clog the shearing assembly so far, but we've noticed it on time (leaking) and then washed it. No problems occurred after that.
It might be that your shearing assembly was clogged but you kept on shearing and the syringe broke.
Try contacting the Digilab technical support as well for more info!Comment
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