RNA Extraction from a Small Number of Bacterial Cells

[megalodon]

Hi,

I am looking for an RNA extraction protocol that can be used for environmental samples that contain very few bacterial cells (~1000). None of the commercially available RNA extraction kits for bacteria that I am aware of are designed for environmental samples (mixture of gram+ and gram- bacteria) and such low cell numbers.

Any help with this would be GREATLY appreciated!

Thanks a lot in advance!

Daniela

[campbellsoup]

Maybe you could try using this?

http://www.invitrogen.com/1/1/15811-picopure-rna-isolation-kit.html