Hello all,
I'm gearing up to make a bunch of libraries using Illumina's TruSeq HT Stranded mRNA kit. I went with the HT kits based upon the number of samples I'm going to be processing. Looking through the protocol, I've noticed a bunch of the temperature incubation steps call for the use of a microheating system, instead of a thermocycler. One reason for this appears to be that the protocol calls for the use of flat-bottomed MIDI plates during these steps. I'm wondering if anyone has had any success with the HT protocol using a thermocycler instead of the microheating system? This of course implies switching to 96-well plates having conical bottoms, instead of the flat-bottom. Thanks in advance for your help.
Cheers
I'm gearing up to make a bunch of libraries using Illumina's TruSeq HT Stranded mRNA kit. I went with the HT kits based upon the number of samples I'm going to be processing. Looking through the protocol, I've noticed a bunch of the temperature incubation steps call for the use of a microheating system, instead of a thermocycler. One reason for this appears to be that the protocol calls for the use of flat-bottomed MIDI plates during these steps. I'm wondering if anyone has had any success with the HT protocol using a thermocycler instead of the microheating system? This of course implies switching to 96-well plates having conical bottoms, instead of the flat-bottom. Thanks in advance for your help.
Cheers